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kinB [2017-07-24 12:40:26]
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kinB [2017-07-24 12:40:26]

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, senses changes in respiratory activity
Locus
BSU31450
Isoelectric point
6.68
Molecular weight
47.71 kDa
Protein length
429 aa Sequence Blast
Gene length
1287 bp Sequence Blast
Function
initiation of sporulation
Product
two-component sensor kinase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,230,067 → 3,231,353

The protein

Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of Spo0F
  • mainly active in the older, inner regions of a colony (with KinA) PubMed
  • sensing potassium level in the medium during initiation of sliding PubMed
  • Domains

  • six transmembrane segments, C-terminal histidine phosphotransferase domain
  • selectivity filter sequence of potassium channels that is required for sliding but not for sporulation PubMed
  • Modification

  • autophosphorylation on a His residue
  • Effectors of protein activity

  • activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus PubMed
  • Structure

  • 3D36 (from G. stearothermophilus, complex with Sda)
  • Localization

  • cell membrane (integral membrane protein) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • stringent response: positive regulation, PubMed, in stringent response
  • SinR: repression, PubMed, in SinR regulon
  • Regulation

  • induced upon addition of decoyinine (positive stringent response) PubMed
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    Biological materials

    Mutant

  • JH19980 (kinB::tet) PubMed
  • References

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